Antibiotic Resistance and Plasmid Profiles of Vibrio Isolates from Muthupettai Mangrove Environment, Southeast Coast of India

A total of 209 pathogenic vibrios strains were isolated from Muthupettai mangrove environment, Southeast coast of India. All strains were identified to be 8 species (Vibrio cholera, Vibrio harveyi, Vibrio mimicus, Vibrio splendidus, Vibrio aestuarianus, Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio metschnikovii) from all stations. The levels of resistance of bacteria to various antibiotics differed considerably. Among these 3 species (13 strains) multiple antibiotic resistance bacteria were identified from all isolates such as Vibrio cholerae (5 strains), Vibrio parahaemolyticus (5 strains) and Vibrio vulnificus (3 strains). The all strains were also able to resistance concentration of antibiotics up to 150μg/ml. The isolated strains were screened for plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antibiotics by the agar dilution method. 13 strains belonging to 3 species have been found to Muthupettai mangroves 1 – 3 plasmids, with sizes ranging from 11 – 112kb. (J Int Dent Med Res 2010; 3: (1), pp. 38-44 )